Integrated DNA Technologies (IDT) has launched oPoolsOligo Pools, a ready-to-use pools of high-quality DNA oligonucleotides (oligos) up to 350 nucleotides in length, as part of its mission to advance biological research worldwide.

IDT has developed new oPools,the turnkey, affordable pools of customised single-stranded DNA sequences, leveraging its 30 years of dedication to exceptional quality products, and its advanced oligo manufacturing processes.

IDT said that its oPoolsoligo pools offer uniform yields to accelerate experiments and eliminate the timeconsuming PCR amplification steps required by other commercially available oligo pools.

IDT president Trey Martin said: “IDT has historically avoided the traditional pools market due to our capacity being fully booked. Two years into our 6th-generation synthesis platform, we have alleviated all capacity concerns and are thrilled to offer our unique take on oligo pools.

“They leverage the ultra-high fidelity of IDT synthesis to give heretofore unobtainable experimental results from the traditional microarray-driven approach. We have been in beta testing for six months and customers are delighted with the results.”

IDT synthesises high-fidelityoligosoPools

Pooled oligos, which are extensively used in high-throughput workflows in synthetic biology, diagnostic development, and drug discovery, are required to be with uniform full-length oligos of the highest possible fidelity, (being in correct sequence and length).

IDTemploys a unique solid-support manufacturing platform, to produce long oligos more accurately, rapidly, and reliably, compared to other DNA synthesis platform, resulting in timely delivery of the uniform oligonucleotide pools.

In addition, it produces oPoolswith a low error rate of less than one in 2,000 nucleotides, and high per oligo yields, that allows researchers to proceed directly to downstream workflows for projects including CRISPR library construction, protein screening, and gene assembly.

Martin added: “Our pool deliveries are in picomoles, eliminating the need to do any pre-amplification or other pre-work before the pool can be used. With lengths up to 350 bases and error rates below one per 2kB, these are the same oligos that IDT relies on for our own high-throughput gene assembly processes.”